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HelpTest Code THS T-Cell Subsets, Blood
Performing Laboratory
North Shore University Hospital Manhasset
Methodology
Flow Cytometry
Includes CD3, CD4,CD8, CD19, CD1656, ABS CD3, ABS CD4, ABS CD8, AB CD19, ABS CD1654 and 4/8 Ratio
Reference Values
|
|
CD3 |
CD4 |
CD8 |
CD19 |
CD1656 |
ABS CD3 |
ABS CD4 |
ABS CD8 |
ABS CD19 |
ABS CD1656 |
4/8 Ratio |
|
Age |
% |
% |
% |
% |
% |
cells/μl |
cells/μl |
cells/μl |
cells/μl |
cells/μl |
|
|
0-3 mo |
53-84 |
35-64 |
12-28 |
6-32 |
4-18 |
2500-5500 |
1600-4000 |
560-1700 |
300-2000 |
170-1110 |
NA |
|
3-6 mo |
51-77 |
35-56 |
12-23 |
11-41 |
3-14 |
2500-5600 |
1800-4000 |
590-1600 |
430-3000 |
170-830 |
NA |
|
6-12 mo |
49-76 |
31-56 |
12-24 |
14-37 |
3-15 |
1900-5900 |
1400-4300 |
500-1700 |
610-2600 |
160-950 |
NA |
|
1-2 yr |
53-75 |
32-51 |
14-30 |
16-35 |
3-15 |
2100-6200 |
1300-3400 |
620-2000 |
720-2600 |
180-920 |
NA |
|
2-6 yr |
56-75 |
28-47 |
16-30 |
14-33 |
4-17 |
1400-3700 |
700-2200 |
490-1300 |
390-1400 |
130-720 |
NA |
|
6-12 yr |
60-76 |
31-47 |
18-35 |
13-27 |
4-17 |
1200-2600 |
650-1500 |
370-1100 |
270-860 |
110-480 |
NA |
|
12-18 yr |
56-84 |
31-52 |
18-35 |
6-23 |
3-22 |
1000-2200 |
530-1300 |
330-920 |
110-570 |
70-480 |
NA |
|
18-65 yr |
59-83 |
30-62 |
12-36 |
6-24 |
5-23 |
672-1870 |
489-1457 |
142-740 |
84-469 |
71-410 |
0.90-3.60 |
|
66-150 yr |
58-84 |
30-56 |
11-43 |
4-18 |
7-27 |
396-2024 |
325-1251 |
90-775 |
32-326 |
80-426 |
0.86-4.14 |
Reference ranges for pediatric population (0-18 years old) are from Shearer, William T., et al. "Lymphocyte subsets in healthy children from birth through 18 years of age: the Pediatric AIDS Clinical Trials Group P1009 study." Journal of Allergy and Clinical Immunology 112.5 (2003): 973-980.
Reference ranges for adult (18-65 years old) and geriatric (65 years old and above) populations were developed at North Shore University Hospital at Manhasset, New York.
Test Classification and CPT Coding
86359 - t cells, total count
86360 - CD4 and CD8 plus ratio
Specimen Requirements
Specimen must arrive within 24 hours of draw. Contact the Tumor Marker (Flow Cytometry) Laboratory at 516-562-4169 for collection instructions and consultation on selection of test(s).
Container/Tube: Lavender-top (EDTA) tube
Specimen: 3 mL of EDTA whole blood
Transport Temperature: Room Temperature
Collection Instructions: Forward promptly. Specimen cannot be refrigerated.
Rejection Criteria
All Peripheral Blood samples that were not kept and/or transported at room temperature 18-25°C will be rejected. These samples are also rejected if the laboratory cannot feasibly complete testing within 30 hours post collection and the sample has not been preserved, unless viability is greater than 80%.
Day(s) and Time(s) Performed
Laboratory is open Mondays through Fridays 7:00AM to 12:00AM. All testing is available during these hours. Laboratory is closed on Saturdays, Sundays and Legal Holidays – New Year’s, Martin Luther King Jr, President’s, Memorial, Independence, Labor, Thanksgiving and Christmas Day.
Computer Interface Code
PDM # 5201208