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Test Code THS T-Cell Subsets, Blood

Performing Laboratory

North Shore University Hospital  Manhasset

Methodology

Flow Cytometry

Includes CD3, CD4,CD8, CD19, CD1656, ABS CD3, ABS CD4, ABS CD8, AB CD19, ABS CD1654 and 4/8 Ratio

Reference Values

 

CD3

CD4

CD8

CD19

CD1656

ABS CD3

ABS CD4

ABS CD8

ABS CD19

ABS CD1656

4/8 Ratio

Age

%

%

%

%

%

cells/μl

cells/μl

cells/μl

cells/μl

cells/μl

 

0-3 mo

53-84

35-64

12-28

6-32

4-18

2500-5500

1600-4000

560-1700

300-2000

170-1110

NA

3-6 mo

51-77

35-56

12-23

11-41

3-14

2500-5600

1800-4000

590-1600

430-3000

170-830

NA

6-12 mo

49-76

31-56

12-24

14-37

3-15

1900-5900

1400-4300

500-1700

610-2600

160-950

NA

1-2 yr

53-75

32-51

14-30

16-35

3-15

2100-6200

1300-3400

620-2000

720-2600

180-920

NA

2-6 yr

56-75

28-47

16-30

14-33

4-17

1400-3700

700-2200

490-1300

390-1400

130-720

NA

6-12 yr

60-76

31-47

18-35

13-27

4-17

1200-2600

650-1500

370-1100

270-860

110-480

NA

12-18 yr

56-84

31-52

18-35

6-23

3-22

1000-2200

530-1300

330-920

110-570

70-480

NA

18-65 yr

59-83

30-62

12-36

6-24

5-23

672-1870

489-1457

142-740

84-469

71-410

0.90-3.60

66-150 yr

58-84

30-56

11-43

4-18

7-27

396-2024

325-1251

90-775

32-326

80-426

0.86-4.14

 

 

Reference ranges for pediatric population (0-18 years old) are from Shearer, William T., et al. "Lymphocyte subsets in healthy children from birth through 18 years of age: the Pediatric AIDS Clinical Trials Group P1009 study." Journal of Allergy and Clinical Immunology 112.5 (2003): 973-980.

Reference ranges for adult (18-65 years old) and geriatric (65 years old and above) populations were developed at North Shore University Hospital at Manhasset, New York.

Test Classification and CPT Coding

86359 - t cells, total count

86360 - CD4 and CD8 plus ratio

Specimen Requirements

Specimen must arrive within 24 hours of draw. Contact the Tumor Marker (Flow Cytometry) Laboratory at 516-562-4169 for collection instructions and consultation on selection of test(s).

 

Container/Tube: Lavender-top (EDTA) tube

Specimen:  3 mL of EDTA whole blood

Transport Temperature:  Room Temperature

Collection Instructions:  Forward promptly. Specimen cannot be refrigerated.

Rejection Criteria

All Peripheral Blood samples that were not kept and/or transported at room temperature 18-25°C will be rejected. These samples are also rejected if the laboratory cannot feasibly complete testing within 30 hours post collection and the sample has not been preserved, unless viability is greater than 80%.

 

Day(s) and Time(s) Performed

Laboratory is open Mondays through Fridays 7:00AM to 12:00AM. All testing is available during these hours. Laboratory is closed on Saturdays, Sundays and Legal Holidays – New Year’s, Martin Luther King Jr, President’s, Memorial, Independence, Labor, Thanksgiving and Christmas Day.

Computer Interface Code

PDM #  5201208