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Test Code SHIGA TOXIN Enterohemorrhagic E coli (EHEC) Shiga Toxin, EIA

Methodology

Detection of enterohemorrhagic E coli Shiga toxins by enzyme immunoassay (EIA)

Performing Laboratory

Laboratory Corporation of America

Specimen Required

Container/Tube: Stool culture transport vial; culture collection swab may be used to collect rectal swabs or a swab of fecal material, then swab should be placed in stool culture transport vial (Carey-Blair C/S media).

Specimen:  1-2 g placed in stool transport vial  ( Rectal swab with obvious stool -min)

Transport Temperature:   Room Temperature

 

Causes for Rejection

Specimen received in grossly leaking transport container; diapers; dry specimen; specimen submitted in fixative or additive; specimen received in expired transport media or incorrect transport device; inappropriate specimen transport conditions (not in a C&S vial or in an overfilled C&S vial); specimen received after prolonged delay in transport (usually more than 72 hours); specimen stored or transported frozen; wooden shaft swab in transport device; unlabeled specimen or name discrepancy between specimen and request label

 

Reference Values

No enterohemorrhagic E coli Shiga toxin detected

 

There are four types of pathogenic E coli (see table). This test detects only enterohemorrhagic E coli; tests are not available for the other pathogenic E coli.

 

Four Major Categories of Diarrheagenic E coli

Category

Clinic Manifestation

Enterotoxigenic (ETEC)

Travelers' diarrhea and infant diarrhea in less developed countries

Enteropathogenic (EPEC)

Infant diarrhea

Enterohemorrhagic (EHEC)

Hemorrhagic colitis

Hemolytic uremic syndrome

Thrombotic thrombocytopenia purpura

Enteroinvasive (EIEC)

Dysentery

Test Classification and CPT Coding

87427

 

LONIC code: 21262-1

Computer Interface Code

6400035

Used For

Detect the presence of Shiga-toxin-producing enterohemorrhagic E coli

 

Limitations

There are four types of pathogenic E coli (see table). This test detects only enterohemorrhagic E coli; tests are not available for the other pathogenic E coli.

Four Major Categories of Diarrheagenic E coli

Category

Clinic Manifestation

Enterotoxigenic (ETEC)

Travelers' diarrhea and infant diarrhea in less developed countries

Enteropathogenic (EPEC)

Infant diarrhea

Enterohemorrhagic (EHEC)

Hemorrhagic colitis

Hemolytic uremic syndrome

Thrombotic thrombocytopenia purpura

Enteroinvasive (EIEC)

Dysentery