Lymphocyte Mitogen Prolif Lymphocyte Mitogen Prolif

Synonyms

Allscripts (AEHR) Order Name

Lymphocyte Mitogen Stimulation Assay

Sunrise Clinical Manager (SCM) Order Name

Lymphocyte Mitogen Stimulation Assay

Clinical Info

Assessing T-cell function in patients on immunosuppressive therapy, including solid-organ transplant patients
Evaluating patients suspected of having impairment in cellular immunity
Evaluation of T-cell function in patients with primary immunodeficiencies, either cellular (DiGeorge syndrome, T-negative severe combined immunodeficiency: SCID, etc) or combined T- and B-cell immunodeficiencies (T- and B-negative SCID, Wiskott Aldrich syndrome, ataxia telangiectasia, common variable immunodeficiency, among others) where T-cell function may be impaired
Evaluation of T-cell function in patients with secondary immunodeficiency, either disease related or iatrogenic
Evaluation of recovery of T-cell function and competence following bone marrow transplantation or hematopoietic stem cell transplantation

Specimen Type

Blood

Container

Green Top Tube

Collection Instructions

NOTE: DO NOT COLLECT FRIDAY, SATURDAY AND SUNDAYS
Container/Tube: Dark Green (Sodium Heparin) Tube
Specimen:
Less than 3 months: 1 mL
3 months-5 years: 2 mL
6-18 years: 3 mL
Greater than 18 years: 10 mL
Minimum Volume: 1 mL whole blood
Stability: 24 Hrs Room Temperature
Collection Instructions: Send specimen is original tube. Do not aliquot.

Transport Instructions

Specimen Stability

24 Hrs Room Temperature

Methodology

Flow Cytometry

Days Performed

Monday through Friday

Performing Laboratory

Mayo Medial Laboratories
NOTE: DO NOT COLLECT FRIDAY, SATURDAY AND SUNDAYS

CPT

86353
86353 (if appropriate)
LOINC Code: 69018-0

PDM

5901660

Result Interpretation

Viability of lymphocytes at day 0: ≥75.0%

Maximum proliferation of phytohemagglutinin as % CD45:
≥49.9%

Maximum proliferation of phytohemagglutinin as % CD3:
≥58.5%

Maximum proliferation of pokeweed mitogen as % CD45:
≥4.5%

Forms