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Test Code LEGIPCR Legionella species, Molecular Detection, PCR, Varies

Important Note

Mayo test code: LEGRP

Reporting Name

Legionella PCR

Useful For

Sensitive and rapid diagnosis of pneumonia caused by Legionella species


The assay is not recommended as a test of cure because bacteria nucleic acids may persist after successful treatment.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Necessary Information

Specimen source is required.

Specimen Required

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Legionella DNA is unlikely.


Specimen Type: Respiratory

Sources: Sputum, tracheal secretions/aspirates, transtracheal aspirate, bronchial washing/aspirate, bronchoalveolar lavage, lung fluid or pleural fluid

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Type: Fresh tissue or biopsy

Sources: Lung

Container/Tube: Sterile container

Specimen Volume: Entire collection

Specimen Minimum Volume

Fluid: 0.5 mL
Tissue: 5 mm(3)

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
LEGRP Legionella PCR 5020-3


Result ID Test Result Name Result LOINC Value
SRC57 Specimen Source 31208-2
29515 Result 5020-3

Clinical Information

Legionnaires disease was first recognized during a pneumonia outbreak at the Legionnaires convention in Philadelphia in 1976. Investigators with the CDC isolated a novel, gram-negative bacillus, later named Legionella pneumophila. It is now widely recognized that L pneumophila (and other members of the genus Legionella) cause Legionnaires disease.

Reject Due To

Tissue/Other Tissue in formalin, formaldehyde, or acetone; formalin-fixed paraffin-embedded (FFPE) block

NY State Approved


Method Name

Rapid Polymerase Chain Reaction (PCR)

Analytic Time

3 days


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Northwell Health Laboratories Additional Information:

Computer Interface Code

    PDM #  1559868