Synonyms
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Allscripts (AEHR) Order Name
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Kaolin Clotting Time
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Sunrise Clinical Manager (SCM) Order Name
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Kaolin Clotting Time
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Clinical Info
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The KCT is a sensitive screening test of lupus anticoagulants (LA) in patients with a history of thrombosis.
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Specimen Type
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Blood
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Container
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Blue Top Tube
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Collection Instructions
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Container/Tube: Light Blue tube 3.2 % buffered sodium citrate
Specimen: 1 ml citrated plasma( 0.5 mL min)
Transport Temperature: Frozen
Collection
Citrated plasma samples should be collected by double centrifugation.
Blood should be collected in a blue-top tube containing 3.2% buffered
sodium citrate. Evacuated collection tubes must be filled to completion
to ensure a proper blood-to-anticoagulant ratio. The sample should be
mixed immediately by gentle inversion at least six times to ensure adequate
mixing of the anticoagulant with the blood. A discard tube is not required prior
to collection of coagulation samples except when using a winged blood
collection device (ie, "butterfly"), in which case a discard tube should be used.
When noncitrate tubes are collected for other tests, collect sterile and nonadditive
(red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate
anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and
serum tubes with clot initiators should also be collected after the citrate tubes.
Centrifuge and carefully remove the plasma using a plastic transfer pipette,
being careful not to disturb the cells.
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Transport Instructions
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Frozen
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Specimen Stability
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4 hours Room Temperature
7 Days Frozen
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Methodology
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Clot
In the kaolin clotting time (KCT) test, kaolin, a negatively-charged
particulate activator, is incubated with test plasma to activate the
contact factors and the intrinsic system. Calcium chloride is added
and the mixture is timed until a clot forms.
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Days Performed
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Monday and Wednesday
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Performing Laboratory
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LabCorp
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CPT
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85347
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PDM
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5900319
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Result Interpretation
50 - 130 units
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Forms
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