Test Code JAK12 JAK2 Exons 12 Mutations, Qualitative, Leumeta®
Performing Laboratory
Quest Diagnostics Nicols Institute, Chantilly, VA
Methodology
Polymerase Chain Reaction-based DNA Sequencing
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Reference Values
Mutations: Not Detected
Test Classification and CPT Coding
81403
Specimen Required
Container/Tube: Lavender EDTA tube
Specimen: 5 ml Whole Blood from EDTA Lavender top
tube(1 mL min) or 1 mL min Bone Marrow(0.5 mL min))
Alternative Specimen(s)
5 mL whole blood or 1 mL bone marrow aspirate collected in: sodium heparin (green-top) tube • Fixed cell pellets • Extracted DNA from CLIA-certified laboratory
Transport Temperature: Refrigerated.
Stability: Room temperature: 7 days
Refrigerated: 7 days
Frozen:
Unacceptable
Note: Ship immediately to maintain sample stability. Stable for only 72 hours room temperature.
Day(s) and Time(s) Performed
Monday - Friday
Analytical time 3 days
Computer Interface Code
PDM # 5907501
Useful For
This DNA-based assay tests leukocytes from blood or bone marrow aspirate for mutations in exon 12 of JAK2, using an advanced DNA sequencing method. These mutations are associated with myeloproliferative neoplasms, particularly polycythemia vera