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Test Code HIVINTEGRASE HIV-1 Integrase Genotype

Performing Laboratory

Quest Infectious Disease, Inc.
33608 Ortega Highway
San Juan Capistrano, CA 92690-6130

Methodology

Sequencing

Reverse Transciptase Polymerase Chain Reaction (RT-PCR)

Specimen Required

Container/Tube: Lavender EDTA or PPT potassium EDTA(white top) tube

Specimen:  2 mL plasma (0.6 mL min)

Transport Temperature:  Frozen

 

Note:  Value of last viral load and date of last viral load must be provided.

Reference Values

See report

 

 

Major Mutations Associated with Resistance to Integrase Strand Transfer Inhibitors
Drug Major Mutations
Raltegravir N155H/S, Q148H/K/R, Y143C/H/R3,4,5
Elvitegravir N155H/S, Q148H/K/R, E92Q/G, T66A/I/K, S147G6,7
Dolutegravir Q148H/K/R, G140A/C/S, E138A/K/T, L74I8–11

 

Test Classification and CPT Coding

87906

Useful For

The emergence of integrase drug resistance mutations has been observed in vitro and in patients experiencing virologic failure on raltegravir in clinical trials. Twenty three percent of patients receiving raltegravir in a clinical trial experienced virologic failure at 48 weeks and genotypic analysis detected raltegravir associated resistance mutations in 68% of virologic failures. This assay amplifies and sequences the HIV-1 integrase gene and reports mutations at positions associated with integrase inhibitor drug resistance.

 

 

Clinical Use

  • Guide selection of antiretroviral drugs

  • Predict HIV-1 resistance to the integrase strand transfer inhibitors (INSTIs) raltegravir (Isentress®), elvitegravir (a component of Stribild™), and dolutegravir (Tivicay®)

  • Monitor transmission of integrase inhibitor-resistant HIV-1

 

Computer Interface Code

PDM #  5950973