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Test Code F2ISO F2-Isoprostanes, Random, Urine

Reporting Name

F2-Isoprostanes, U

Useful For

The assessment of in vivo lipid peroxidation and considered to be an index of systemic oxidative stress over time 

Additional Tests

Test ID Reporting Name Available Separately Always Performed
AACT Creatinine, U No Yes

Testing Algorithm

When F2-Isoprostanes testing is performed, urine creatinine will always be performed at no additional charge.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Specimen Required

Patient Preparation: Patient should not have taken nonsteroidal anti-inflammatory drugs within 72 hours or aspirin within 2 weeks prior to collection of a specimen.

Container/Tube: Plastic, 5-mL urine tube (T465)

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 7 days
  Frozen  90 days
  Ambient  7 days

Reference Values

≥18 years: ≤1.0 ng/mg creatinine

<18 years: not established

Day(s) and Time(s) Performed

Wednesday; 11 a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
F2ISO F2-Isoprostanes, U 90783-2


Result ID Test Result Name Result LOINC Value
88677 15-F2t-Isoprostane, U 90783-2

Clinical Information

Oxidative stress results from the generation and overaccumulation of reactive oxygen and nitrogen species and has been shown to damage lipoproteins, lipids, DNA, and proteins. Furthermore, oxidative stress may modulate modifications to these lipoproteins and DNA such that endothelial function and inflammatory processes are altered, ultimately resulting in the initiation and progression of atherosclerosis and cardiovascular disease (CVD). Isoprostanes are a series of prostaglandin-like compounds produced via the free-radical catalyzed peroxidation of arachidonic acid, independent of the cyclooxygenase-derived prostaglandins. F2-isoprostanes are considered the "gold standard" test for quantifying lipid peroxidation/oxidative stress in vivo. 15-F2t-isoprostane (15-F2t-IsoP), also referred to as 8-iso-PGF2 alpha or 8-isoprostane F2 alpha, is 1 of the F2-isoprostanes produced in abundance in vivo and has demonstrated potency as a vasoconstrictor within the vasculature of the heart, brain, lung, and kidneys. Generation of 15-F2t-IsoP induces downstream effects including proliferation of vascular smooth muscle cells and release of endothelin. Additional evidence suggests that F2-isoprostanes may increase aspirin resistance to platelet aggregation within platelets and whole blood.


F2-isoprostanes are advantageous over other markers of lipid peroxidation due to their in vivo and in vitro stability and are detectable in a variety of human tissues and biological fluids including plasma, urine, lavage fluid, RBCs, and cerebrospinal fluid. Quantitation of F2-isoprostanes in a random urine specimen is considered to be the most accurate and robust measurement of circulating isoprostanes and is a noninvasive method of assessment.

Analytic Time

2 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

NY State Approved


Method Name

F2ISO: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

AACT: Enzymatic Colorimetric Assay


If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen.