Test Code DNSAG Dengue Virus NS1 Antigen, Serum
Specimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Useful For
Aiding in the diagnosis of dengue virus infection
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
DENS1 | Dengue NS1 Ag, S | No | Yes |
DNAGI | Dengue Ag Interpretation | No | Yes |
Testing Algorithm
See Mosquito-borne Disease Laboratory Testing in Special Instructions.
Special Instructions
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Dengue Virus NS1 Ag, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 14 days | |
Frozen | 14 days |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | Reject |
Clinical Information
Dengue virus (DV) is a globally distributed flavivirus with 4 distinct serotypes (DV-1, -2, -3, -4) and is primarily transmitted by the Aedes aegypti mosquito, found throughout the tropical and subtropical regions of over 100 countries. DV poses a significant worldwide public health threat with approximately 2.5 to 3 billion people residing in DV endemic areas, among whom 100 to 200 million individuals will be infected, and approximately 30,000 patients will succumb to the disease, annually.
Following dengue infection, the incubation period varies from 3 to 7 days, and while some infections remain asymptomatic, the majority of individuals will develop classic dengue fever. Symptomatic patients become acutely febrile and present with severe musculoskeletal pain, headache, retro-orbital pain, and a transient macular rash, most often observed in children. Fever defervescence signals disease resolution in most individuals. However, children and young adults remain at increased risk for progression to dengue hemorrhagic fever and dengue shock syndrome, particularly during repeat infection with a new DV serotype.
Detection of the DV nonstructural protein 1 (NS1) has emerged as an alternative biomarker to both serologic and molecular based techniques for diagnosis of acute DV infection. NS1 antigenemia is detectable within 24 hours and up to 9 days following symptoms onset. This overlaps with the DV viremic phase and NS1 is often detectable prior to IgM seroconversion. Concurrent evaluation for the NS1 antigen alongside testing for IgM- and IgG-class antibodies to DV (DENGM / Dengue Virus Antibody, IgG and IgM, Serum) provides optimal diagnostic potential for both early and late dengue disease.
Reference Values
Negative
Reference values apply to all ages.
Day(s) and Time(s) Performed
Monday; 8 a.m.
Analytic Time
Same day/1 dayPerforming Laboratory

Test Classification
This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86790-NS1 Ag
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
DNSAG | Dengue Virus NS1 Ag, S | 75377-2 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
DENS1 | Dengue NS1 Ag, S | 75377-2 |
DNAGI | Dengue Ag Interpretation | 69048-7 |