ADAMTS13 Assay ADAMTS13

Synonyms

Allscripts (AEHR) Order Name

ADAMTS13 Activity Reflex

Sunrise Clinical Manager (SCM) Order Name

ADAMTS 13 Activity Reflex

Clinical Info

Diagnostic for  Thrombotic Thrombocytopenic Purpura (TTP) 

Specimen Type

Blood

Container

Blue Top Tube

Collection Instructions

Specimen:  1.0 mL citrated plasma (0.5 mL min)
        Limitations: Samples containing EDTA cannot be used because EDTA is a strong inhibitor of ADAMTS13 function.
Hemolysis does not affect assay performance with samples containing up to 200mg/dL of hemoglobin.
Lipemia does not affect assay performance with samples containing up to 300mg/dL of lipids.
Icterus does not affect assay performance with samples containing up to 15mg/dL of bilirubin (both conjugated and unconjugated).

Transport Instructions

Frozen 

Specimen Stability

7 Days Room Temperature
7 Days Refrigerated
2 Weeks Frozen

Methodology

Chromogenic ELISA 

Days Performed

TAT: 1- 3 Days

Performing Laboratory

Machaon Diagnostics

CPT

85397
85397, 85335 if Inhibitor is performed
83520 is Antibody performed

PDM

5910042

Result Interpretation

ADAMTS13 Activity:  40-130%

ADAMTS13 Inhibitor:  Negative

ADAMTS13 Ab : <12 U/ml

 Note:  ADAMTS13 activity reflex to ADAMTS13 Inhibitor when activity is less than 40%. If ADAMTS13 Inhibitor is Positive the ADAMTS13 Antibody  will sreflex

Forms