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Test Code ACTHSTIM ACTH Stimulation Test Panel

Performing Laboratory

Northwell Health Laboratories


Electrochemiluminescence Immunoassay (ECIA)

Reference Values

See individual tests


The test report will include the following interpretive comment:

Morning (preferably around 8 AM) specimens are required. The ACTH stimulation test requires expert administration and interpretation.*


*Burgos N, Ghayee H,Singh-Ospina N [June 2019] Pitfalls in the interpretation of the cosyntropin stimulation test for the diagnosis of adrenal insufficiency, Current Opinion in Endocrinology & Diabetes and Obesity; 26,3:139-145 doi: 10.1097/MED.000000000000047

Test Classification and CPT Coding


82533 x 3

Specimen Requirements


Tube Label




Transport Temperature

ACTH Baseline

Before injection

Lavender-top tube

1.5 mL of EDTA plasma (0.3 mL minimum)

separate and

freeze plasma ASAP

Frozen: specimen is not stable at ambient temperature

Cortisol Baseline

Before injection

Gold Top tube

1 mL of serum

(0.3 mL minimum)
Centrifuge and separate


Cortisol 30 min

30 min after injection

Cortisol 60 min

60 min after injection

Record the date and time of collection on the tube and, if not indicated, the test names

ACTH Baseline, Cortisol Baseline, Cortisol 30 min, or Cortisol 60 min


This test cannot be performed at a Patient Service Center because it requires intravenous injection by a physician or other qualified medical professional.

Computer Interface Code

PDM #'s

 ATCH Baseline        1953204

Cortisol Baseline       1953205

Cortisol 30 minutes   1953206

Cortisol 60 minutes  1953207


Used For

The adrenocorticotropic hormone (ACTH) stimulation procedure is for the evaluation of adrenal function.