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Test Code 5HIAA 5-Hydroxyindoleacetic Acid (HIAA) 24 urine with creatinine

Additional Codes

Sunrise Clinical Manager (SCM) Orderable:   5HIAA w/Creatinine 24 hr UR

AllScripts (AEHR) Orderable:  5HIAA w/Creatinine 24 hr UR

Performing Laboratory

Quest Diagnostics Nichols Institute, Chantilly VA


High Performance Liquid Chromatography (HPLC)

Specimen Required

Container/Tube: 24 hours urine container


10 mL aliquot  of a 24-hour urine collected with 25 mL of 6N HCl (5 mL min)

  Urine without preservative is acceptable if pH is below 6.

Transport Temperature: Refrigerated

Stability: 7 Days Room Temperature

               30 Days Refrigerated


 Keep urine refrigerated during collection if preservative is not used. Record 24-hour urine volume on test requesition and the urine vial.

Patient Preparation:

Patient should avoid food high in indoles: avocados, banana, tomato, plum, walnut, pineapple, and eggplant. Patient should also avoid tobacco, tea and coffee three days prior to specimen collection.



Reference Values

2-10 years: 8.0 mg/24 hr or less
>10 years: 6.0 mg/24 hr or less


Limitations: Patients with renal disease may display falsely decreased results. Urinary 5-HIAA is increased in patients with malabsorption disorders who also display a larger concentration of urinary tryptophan metabolites. Urinary 5-HIAA is increased in patients with intestinal obstruction and with some noncarcinoid islet cell tumors. Carcinoid syndrome may not cause elevated results, especially if the patient does not have diarrhea. Requires accurate 24-hour urine collection.

Test Classification and CPT Coding




TAT - 3 - 4 Days

Computer Interface Code

PDM # 5901716

Useful For

Values above 10 mg/24 hours occur in carcinoid syndrome. Slight elevations also occur in nontropical sprue and after reserpine administration. Drugs which can interfere with testing due to physiologic response: acetaminophen, alpha and beta blockers, atenolol, bromocriptine, broncholdilators, clonidine, digoxin, isoniazid, L-dopa, labetelol, methyldopa, monoamine oxidase inhibitors, nitroglycerin, sympathomimetic amines, phenobarbital, phenothiazines, phentolamine, reserpine, salicylates, tricyclic antidepressants.