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Test Code 23BPG 2,3-Dinor-11Beta-Prostaglandin F2 Alpha, Urine

Useful For

Screening for mast cell activation disorders including systemic mastocytosis

Testing Algorithm

When this test is performed, urine creatinine will always be performed at no additional charge.

Method Name

23BPG: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

AACT: Enzymatic Colorimetric Assay

Reporting Name

2,3-dinor 11B-Prostaglandin F2a, U

Specimen Type


Advisory Information


Specimen Required

Submit only 1 of the following specimens:


Patient Preparation: Patients taking aspirin or nonsteroidal anti-inflammatory drugs (NSAIDs) may have decreased concentrations of prostaglandin F2 alpha. If possible, discontinue for 2 weeks or 72 hours, respectively, prior to collecting a specimen.


Preferred: 24-hour urine collection

Supplies: Plastic, 5-mL tube (T465)

Specimen Volume: 4 mL

Collection Instructions:

1. Collect urine for 24 hours.

2. Refrigerate specimen during collection, and send specimen refrigerated.

Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens in Special Instructions for multiple collections.


Acceptable: Random collection

Supplies: Plastic, 5-mL tube (T465)

Specimen Volume: 4 mL

Collection Instructions:

1. Collect a random urine specimen. 

2. Refrigerate specimen after collection. Send specimen refrigerated or frozen; do not add any preservative.

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  8 hours

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Clinical Information

2,3-Dinor-11beta-prostaglandin F2 alpha is the most abundant metabolic product of prostaglandins released by activated mast cells. Systemic mastocytosis (SM) is a disease in which clonally derived mast cells accumulate in peripheral tissues. Degranulation of these mast cells releases large amounts of histamines, prostaglandins, leukotrienes, and tryptase.


The World Health Organization diagnostic criteria for SM require the presence of elevated mast cell counts on a bone marrow biopsy and 1 of the following minor criteria: abnormal mast cell morphology, KIT Asp816Val mutation, CD25-positive mast cells, or serum tryptase greater than 20 ng/mL. Alternatively, SM diagnosis can be made with the presence of 3 minor criteria in the absence of abnormal bone marrow studies.


Measurement of mast cell mediators in blood or urine is less invasive and is advised for the initial evaluation of suspected cases. Elevated levels of serum tryptase, urinary N-methylhistamine (NMH), 2,3-dinor-11beta-prostaglandin F2 alpha (2,3 BPG), or leukotriene E4 (LTE4) are consistent with the diagnosis of systemic mast cell disease.

Reference Values

<5,205 pg/mg creatinine

Day(s) and Time(s) Performed

Monday, Thursday; 11 a.m.

Analytic Time

2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
23BPG 2,3-dinor 11B-Prostaglandin F2a, U 94381-1


Result ID Test Result Name Result LOINC Value
37931 2,3-dinor 11B-Prostaglandin F2a, U 94381-1

NY State Approved


Urine Preservative Collection Options

Note: The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection.







50% Acetic Acid


Boric Acid


Diazolidinyl Urea


6M Hydrochloric Acid


6M Nitric Acid


Sodium Carbonate






Northwell Health Laboratories Additional Information:

Computer Interface Code

    PDM #  1759101