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Immunoglobulin Gene Rearrangement

Performing Laboratory

Northwell Health Laboratories

Methodology

Genomic DNA Extracted Followed by Polymerase Chain Reaction (PCR) or Southern Blot

Reference Values

Pathologist interpretation

Test Classification and CPT Coding

“Pathology Consultation, Limited Without Review of Patient’s Medical Record”

80500

“Immunoglobulin Gene Rearrangement, Polymerase Chain Reaction (PCR)”

83894 x 5 - electrophoresis

83901 x 7 - each multiplex, PCR

“Gene Rearrangement Extraction”

83891 - DNA extract-purify

“Immunoglobulin Gene Rearrangement, Southern Blot” (if appropriate)

83892 x 2 - enzyme digestion

83894 x 2 - electrophoresis

83896 x 2 - nucleic acid probe, each

83897 x 2 - nucleic acid transfer

Day(s) and Time(s) Performed

Monday through Thursday

Physician Office Specimen Requirements

Specimen must arrive within 72 hours of collection.

 

Submit only 1 of the following specimens:

 

Blood

Container/Tube:  Lavender-top (EDTA) tube(s)

Specimen:  6 mL (minimum volume:  3 mL) of EDTA whole blood in original VACUTAINER(S)®

Transport Temperature:  Ambient

Collection Instructions:  Invert several times to mix blood. Forward unprocessed whole blood promptly.

Note:  Include relevant clinical information and cytogenetics results, if available.

 

Bone Marrow

Place 2 mL (minimum volume:  1 mL) of bone marrow in a lavender-top (EDTA) tube(s) and send in original VACUTAINER(S)®. Invert several times to mix bone marrow. Forward unprocessed bone marrow promptly at ambient temperature.

Note:  Include relevant clinical information and cytogenetics results, if available.

 

Solid Tissue

Frozen Tissue

Obtain 200 mg (minimum volume:  100 mg) of fresh tissue. Freeze tissue within 1 hour of collection. Send specimen frozen in plastic container.

Note:  Include relevant clinical information and cytogenetics results, if available.

 

Paraffin-Embedded Tissue

A paraffin block must be sent. (Slide is not acceptable.)

Note:  1. Southern blot testing cannot be performed on paraffin-embedded tissue.

2. Include relevant clinical information and cytogenetics results, if available.

 

Spinal Fluid
5 mL to 10 mL (minimum volume:  1 mL) of spinal fluid in a screw-capped, sterile vial. Maintain sterility and forward promptly at ambient temperature.

Note:  Include relevant clinical information and cytogenetics results, if available.