Synonyms
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Allscripts (AEHR) Order Name
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Mycoplasma Pneumoniae PCR
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Sunrise Clinical Manager (SCM) Order Name
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Mycoplasma pneumoniae PCR
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Clinical Info
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Diagnosing infections due to Mycoplasma (Mycoplasmoides) pneumoniae
Assessing macrolide susceptibility
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Specimen Type
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Body Fluid, CSF, Swab, Respiratory, Nasal, Nasopharyngeal
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Container
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Sterile
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Collection Instructions
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The high sensitivity of amplification by PCR requires the
specimen to be processed in an environment in which contamination
of the specimen by Mycoplasma pneumoniae DNA is unlikely.
Specimen source is required.
Submit only 1 of the following specimens:
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum
Container/Tube: Sterile container
Specimen Volume: 1 mL (0.5 mL min)
Specimen Type: Swab
Specimen: Throat nasal, or nasopharyngeal
Container/Tube: Culture
swab transport system (Dacron or rayon swab with aluminum or plastic shaft with
either Stuart or Amies liquid medium
Acceptable: Culture transport swab (Stuart's media) or
place swab in M4, M4-RT , M5, M6, UTM, or ESwab
Specimen Volume: Swab
Collection Instructions:
1. Collect specimen by swabbing back and forth over mucosa
surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Specimen Type: Fluid
Sources: Pleural, pericardial, cerebrospinal
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Transport Temperature: Refrigerated
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Transport Instructions
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Refrigerated
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Specimen Stability
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Varies
7 days Refrigerated (preferred)
7 days Frozen
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Methodology
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Rapid Polymerase Chain Reaction (PCR) Using Light Cycler and
Fluorescent Resonance Energy Transfer (FRET)
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Days Performed
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TAT 5-6 Days
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Performing Laboratory
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Mayo Medical Laboratories
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CPT
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87581
LOINC Code 29257-3
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PDM
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1759238
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Result Interpretation
Reference Range: Not applicable
Interpretation
A positive result indicates the presence of Mycoplasmoides pneumoniae.
A negative result does not rule out the presence of M pneumoniae and may be due to the presence of inhibitors within the specimen matrix, or the presence of organisms at numbers below the limits of detection of the assay.
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Forms
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