Sign in →

Test Code MATCC Maternal Cell Contamination, Molecular Analysis

Useful For

Ruling out the presence of maternal cell contamination within a fetal specimen

           

This test is required for all prenatal testing performed in Mayo's molecular and biochemical genetics laboratories

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
CULFB Fibroblast Culture for Genetic Test Yes No
CULAF Amniotic Fluid Culture/Genetic Test Yes No
_STR1 Comp Analysis using STR (Bill only) No No
_STR2 Add'l comp analysis w/STR (Bill Only) No No

Testing Algorithm

For prenatal specimens only: If amniotic fluid (nonconfluent cultured cells) is received, amniotic fluid culture/genetic test will be added and charged separately. If chorionic villus specimen (nonconfluent cultured cells) is received, fibroblast culture for genetic test will be added and charged separately.

 

If this test is ordered in conjunction with CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling or CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, and no other molecular testing is ordered, test will be changed to PPAP / Parental Sample Prep for Prenatal Microarray Testing.

Method Name

Polymerase Chain Reaction (PCR)-Based Comparison of Microsatellite Markers

Reporting Name

Maternal Cell Contamination, B

Specimen Type

Varies


Advisory Information


If a prenatal specimen has already been submitted as part of another diagnostic test, a second prenatal specimen is not required. If a prenatal specimen has not yet been submitted, submit only 1 prenatal specimen.



Additional Testing Requirements


A maternal specimen and a prenatal specimen are both required.

-MATCC / Maternal Cell Contamination, Molecular Analysis must be ordered on both the prenatal and maternal specimens under separate order numbers.



Shipping Instructions


Specimen preferred to arrive within 96 hours of collection.



Specimen Required


Patient Preparation: A previous bone marrow transplant from an allogenic donor will interfere with testing. Call Mayo Medical Laboratories for instructions for testing patients who have received a bone marrow transplant.

 

Specimen Type: Maternal blood

Container/Tube:

Preferred: Lavender top (EDTA) or yellow top (ACD)

Acceptable: Any anticoagulant

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Prenatal Specimens:

Submit only 1 of the following specimens:

 

Specimen Type: Cord blood

Container/Tube:

Preferred: Lavender top (EDTA) or yellow top (ACD)

Acceptable: Any anticoagulant

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Specimen Type: Amniotic fluid

Container/Tube: Amniotic fluid container

Specimen Volume: 20 mL

Additional Information: A separate culture charge will be assessed under CULAF / Amniotic Fluid Culture for Genetic Testing.

Specimen Stability Information: Refrigerated (preferred)/Ambient

 

Specimen Type: Cultured amniocytes

Container/Tube: T-25 flask

Specimen Volume: 2 full flasks

Collection Instructions: Submit confluent cultured cells from another laboratory.

Specimen Stability Information: Ambient (preferred)/Refrigerated

Specimen Type: Chorionic villi

Container/Tube: 15-mL tube containing 15 mL of transport media

Specimen Volume: 20 mg

Additional Information: A separate culture charge will be assessed under CULFB / Fibroblast Culture for Genetic Testing.

Specimen Stability Information: Refrigerated

 

Specimen Type: Cultured chorionic villi

Container/Tube: T-25 flasks

Specimen Volume: 2 full flasks

Collection Instructions: Submit confluent cultured cells from another laboratory.

Specimen Stability Information: Ambient (preferred)/Refrigerated


Specimen Minimum Volume

Amniotic Fluid: 10 mL
Blood, Cord Blood: 0.5 mL
Chorionic Villus: 5 mg

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies

Clinical Information

One of the risks associated with prenatal testing is maternal cell contamination (MCC), which can occur when a fetal specimen comes into contact with maternal blood or tissue. The risk of MCC is associated with procedures such as chorionic villus sampling, amniocentesis, or extraction of fetal blood from the umbilical cord (cord blood). If MCC is present, the maternal DNA may mask the results of any genetic testing performed on the fetal DNA. Therefore, the results of prenatal testing may be compromised.

              

To rule out the presence of MCC, a maternal blood specimen is necessary for comparison of maternal and fetal chromosomal markers. The presence of both maternal and nonmaternal alleles for each fetal marker indicates the fetal specimen is not contaminated. MCC is confirmed when both alleles in the fetus are maternal.

Reference Values

An interpretative report will be provided.

Day(s) and Time(s) Performed

Monday; 10 a.m.

Analytic Time

5 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81265-Comparative analysis using Short Tandem Repeat (STR) markers; patient and comparative specimen (eg, pre-transplant recipient and donor germline testing, post-transplant non-hematopoietic recipient germline [eg, buccal swab or other germline tissue sample] and donor testing, twin zygosity testing or maternal cell contamination of fetal cells

 

Fibroblast Culture for Genetic Test

 

88233-Tissue culture, skin or solid tissue biopsy (if appropriate)

 

88240-Cryopreservation (if appropriate)

 

Amniotic Fluid Culture/Genetic Test

 

88235-Tissue culture for amniotic fluid

 

88240-Cryopreservation (if appropriate)

Added as needed:

81266-each additional specimen (eg additional cord blood donor, additional fetal samples from different cultures, or additional zygosity in multiple birth pregnancies)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MATCC Maternal Cell Contamination, B In Process

 

Result ID Test Result Name Result LOINC Value
53285 Result Summary 50397-9
53286 Result 59266-7
53287 Interpretation 69047-9
53288 Reason for referral 42349-1
53289 Specimen 31208-2
53290 Source 31208-2
55150 Method 49549-9
53291 Released By No LOINC Needed

NY State Approved

Conditional

Forms

New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.