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Test Code HLX TCR T-Cell Receptor Gene Rearrangement

Performing Laboratory

Northwell Health Laboratories

Methodology

Polymerase Chain Reaction (PCR)

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Reference Values

Pathologists interpretation

Test Classification and CPT Coding

81340

81342

Physician Office Specimen Requirements

Include relevant clinical information and cytogenetics results, if available.

 

Specimen must arrive within 72 hours of collection.

 

Submit only 1 of the following specimens:

 

Blood

Container/Tube:  Lavender-top (EDTA) tube(s)

Specimen:  5 mL (minimum volume:  3 mL) of EDTA whole blood in original VACUTAINER(S)®

Transport Temperature:  Ambient

Collection Instructions:  Invert several times to mix blood. Forward unprocessed whole blood promptly.

Note:  1. Indicate blood on request form.

2. Label specimen appropriately (blood).

 

Bone Marrow

Obtain 2 mL (minimum volume:  1 mL) of bone marrow in a lavender-top (EDTA) tube(s) and send in original VACUTAINER(S)®. Invert several times to mix bone marrow. Forward unprocessed bone marrow promptly at ambient temperature.

Note:  1. Indicate bone marrow on request form.

2. Label specimen appropriately (bone marrow).

 

Solid Tissue

Frozen Tissue

Obtain 200 mg (minimum volume:  100 mg) of fresh tissue. Freeze tissue within 1 hour of collection. Send specimen frozen in plastic container.

Note:  1. Indicate solid tissue on request form.

2. Label specimen appropriately (solid tissue).

 

Paraffin-Embedded Tissue

A paraffin block must be submitted. (Slide cut from a paraffin block is not acceptable.)

Note:  Southern blot testing cannot be performed on paraffin-embedded tissue.

Note:  1. Indicate paraffin-embedded tissue on request form.

2. Label specimen appropriately (paraffin-embedded tissue).

 

Spinal Fluid
Obtain 5 mL to 10 mL (minimum volume:  1 mL) of spinal fluid in a screw-capped, sterile vial. Maintain sterility and forward promptly at ambient temperature.

Note:  1. Indicate spinal fluid on request form.

2. Label specimen appropriately (spinal fluid).

Day(s) and Time(s) Performed

Monday through Thursday

Computer Interface Code

PDM # 5160176